Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.77455240
Body weight after experiment	0.42371892
Oxygen consumption	0.02474491
Carbon dioxide production	0.21663928
Heat production (metabolic rate)	0.02012236
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.54897857
Cumulative food intake	0.56362302
Respiratory Exchange Ratio	0.00250021
Total water intake	0.56575328
Cumulative water intake	0.21285659
Respiratory Exchange Ratio series	0.00213518
XTOT	0.16980205
XAMB	0.23570663
YTOT	0.70336073
YAMB	0.73645379
ZTOT	0.63799453
ZAMB	0.49552537
Oxygen consumption series	0.03948098
Carbon dioxide production series	0.25971612
Heat production (metabolic rate) series	0.04363664
Total food intake series	0.26014533
Total water intake series	0.30421198
XTOT series	0.05466843
XAMB series	0.06831753
YTOT series	0.51713970
YAMB series	0.53133128
ZTOT series	0.93590707
ZAMB series	0.79998342