Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.66686374
Body weight after experiment	0.15152578
Oxygen consumption	0.95326770
Carbon dioxide production	0.98125014
Heat production (metabolic rate)	0.86044216
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	-
Cumulative food intake	-
Respiratory Exchange Ratio	0.31141924
Total water intake	0.00852663
Cumulative water intake	0.00009971
Respiratory Exchange Ratio series	0.40905793
XTOT	0.56561468
XAMB	0.99200475
YTOT	0.57696683
YAMB	0.75419586
ZTOT	0.31265850
ZAMB	0.24130795
Oxygen consumption series	0.85086455
Carbon dioxide production series	0.91499214
Heat production (metabolic rate) series	0.71497543
Total food intake series	-
Total water intake series	0.03787421
XTOT series	0.28687293
XAMB series	0.76072948
YTOT series	0.50563164
YAMB series	0.82948906
ZTOT series	0.32051382
ZAMB series	0.18768804