Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00260517
Body weight after experiment	0.01303417
Oxygen consumption	0.11349465
Carbon dioxide production	0.08858035
Heat production (metabolic rate)	0.90630144
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.90462362
Cumulative food intake	0.49714093
Respiratory Exchange Ratio	0.65384486
Total water intake	0.95956165
Cumulative water intake	0.40689662
Respiratory Exchange Ratio series	0.68749619
XTOT	0.78366552
XAMB	0.89081525
YTOT	0.49020931
YAMB	0.24648017
ZTOT	0.77756635
ZAMB	0.72196261
Oxygen consumption series	0.07043420
Carbon dioxide production series	0.06568238
Heat production (metabolic rate) series	0.85125389
Total food intake series	0.49789227
Total water intake series	0.64778422
XTOT series	0.77557097
XAMB series	0.75923621
YTOT series	0.54880917
YAMB series	0.23211636
ZTOT series	0.47409355
ZAMB series	0.45849948