Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.52539854
Body weight after experiment	0.24997620
Oxygen consumption	0.84206496
Carbon dioxide production	0.48706996
Heat production (metabolic rate)	0.61898109
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.03617082
Cumulative food intake	0.19673466
Respiratory Exchange Ratio	0.03799973
Total water intake	0.04504337
Cumulative water intake	0.45216968
Respiratory Exchange Ratio series	0.01880938
XTOT	0.98933980
XAMB	0.79187297
YTOT	0.69593879
YAMB	0.71135194
ZTOT	0.31749782
ZAMB	0.35411583
Oxygen consumption series	0.93457172
Carbon dioxide production series	0.53610485
Heat production (metabolic rate) series	0.56716268
Total food intake series	0.65946809
Total water intake series	0.33788624
XTOT series	0.98104579
XAMB series	0.68584926
YTOT series	0.74531734
YAMB series	0.90086805
ZTOT series	0.73226618
ZAMB series	0.63760172