Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.23710605
Body weight after experiment	0.51617729
Oxygen consumption	0.31703791
Carbon dioxide production	0.50364901
Heat production (metabolic rate)	0.94681717
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.34229610
Cumulative food intake	0.10557694
Respiratory Exchange Ratio	0.19296715
Total water intake	0.35037652
Cumulative water intake	0.07418838
Respiratory Exchange Ratio series	0.27371987
XTOT	0.68648042
XAMB	0.97523052
YTOT	0.81414300
YAMB	0.90090787
ZTOT	0.37140928
ZAMB	0.19144719
Oxygen consumption series	0.21917849
Carbon dioxide production series	0.44503562
Heat production (metabolic rate) series	0.89678964
Total food intake series	0.87039427
Total water intake series	0.69621996
XTOT series	0.61658820
XAMB series	0.97171998
YTOT series	0.89302026
YAMB series	0.75031578
ZTOT series	0.34572220
ZAMB series	0.17939939