Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.35254990
Body weight after experiment	0.88987758
Oxygen consumption	0.05395133
Carbon dioxide production	0.22167569
Heat production (metabolic rate)	0.22285991
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.66860358
Cumulative food intake	0.03753528
Respiratory Exchange Ratio	0.03587342
Total water intake	0.86834191
Cumulative water intake	0.65000108
Respiratory Exchange Ratio series	0.04514298
XTOT	0.01632198
XAMB	0.06971587
YTOT	0.02155680
YAMB	0.04686643
ZTOT	0.08489737
ZAMB	0.05828351
Oxygen consumption series	0.07581039
Carbon dioxide production series	0.26067763
Heat production (metabolic rate) series	0.25270337
Total food intake series	0.52806670
Total water intake series	0.19340415
XTOT series	0.00701558
XAMB series	0.02954687
YTOT series	0.00722282
YAMB series	0.01922011
ZTOT series	0.08911268
ZAMB series	0.06660709