Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.03980996
Body weight after experiment	0.36044689
Oxygen consumption	0.00135950
Carbon dioxide production	0.00286982
Heat production (metabolic rate)	0.02410771
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.35150630
Cumulative food intake	0.21291639
Respiratory Exchange Ratio	0.63115301
Total water intake	0.74911298
Cumulative water intake	0.26192887
Respiratory Exchange Ratio series	0.61544530
XTOT	0.36182846
XAMB	0.17934389
YTOT	0.50410249
YAMB	0.31749782
ZTOT	0.22477239
ZAMB	0.54785990
Oxygen consumption series	0.00095153
Carbon dioxide production series	0.00257062
Heat production (metabolic rate) series	0.01880944
Total food intake series	0.75915044
Total water intake series	0.40860107
XTOT series	0.33580381
XAMB series	0.16418302
YTOT series	0.39193879
YAMB series	0.24752122
ZTOT series	0.67130986
ZAMB series	0.98488376