Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.09764962
Body weight after experiment	0.60450039
Oxygen consumption	0.22605387
Carbon dioxide production	0.08871993
Heat production (metabolic rate)	0.02572804
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.96920148
Cumulative food intake	0.91311153
Respiratory Exchange Ratio	0.02908786
Total water intake	0.08132596
Cumulative water intake	0.01260116
Respiratory Exchange Ratio series	0.05319115
XTOT	0.08299149
XAMB	0.03995152
YTOT	0.04469789
YAMB	0.01864653
ZTOT	0.53667338
ZAMB	0.84823543
Oxygen consumption series	0.33211381
Carbon dioxide production series	0.12478914
Heat production (metabolic rate) series	0.04840698
Total food intake series	0.14920481
Total water intake series	0.54334854
XTOT series	0.15558127
XAMB series	0.05176239
YTOT series	0.06401314
YAMB series	0.01839112
ZTOT series	0.51029752
ZAMB series	0.76725142