Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.01598552
Body weight after experiment	0.00918521
Oxygen consumption	0.01403620
Carbon dioxide production	0.03738154
Heat production (metabolic rate)	0.31993579
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.72318672
Cumulative food intake	0.47765156
Respiratory Exchange Ratio	0.64073234
Total water intake	0.02839530
Cumulative water intake	0.99866403
Respiratory Exchange Ratio series	0.62145166
XTOT	0.76115671
XAMB	0.64723023
YTOT	0.53221684
YAMB	0.49981513
ZTOT	0.48294677
ZAMB	0.65885483
Oxygen consumption series	0.03334715
Carbon dioxide production series	0.05620796
Heat production (metabolic rate) series	0.42475773
Total food intake series	0.56066202
Total water intake series	0.17816129
XTOT series	0.93951272
XAMB series	0.87589243
YTOT series	0.50428037
YAMB series	0.36692908
ZTOT series	0.93998769
ZAMB series	0.90187202