Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.23992987
Body weight after experiment	0.48127015
Oxygen consumption	0.00028517
Carbon dioxide production	0.01607370
Heat production (metabolic rate)	0.00020148
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.02395597
Cumulative food intake	0.00698307
Respiratory Exchange Ratio	0.00016635
Total water intake	0.02606317
Cumulative water intake	0.00292609
Respiratory Exchange Ratio series	0.00000339
XTOT	0.81253476
XAMB	0.85161857
YTOT	0.19497048
YAMB	0.15273648
ZTOT	0.43228318
ZAMB	0.35673757
Oxygen consumption series	0.00018442
Carbon dioxide production series	0.01964736
Heat production (metabolic rate) series	0.00006558
Total food intake series	0.60651929
Total water intake series	0.24034152
XTOT series	0.81903734
XAMB series	0.72812602
YTOT series	0.14606338
YAMB series	0.13227545
ZTOT series	0.44878474
ZAMB series	0.61082671