Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.57788041
Body weight after experiment	0.69926116
Oxygen consumption	0.25979874
Carbon dioxide production	0.37182412
Heat production (metabolic rate)	0.18149497
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.21977729
Cumulative food intake	0.07193126
Respiratory Exchange Ratio	0.90512736
Total water intake	0.07029275
Cumulative water intake	0.00823710
Respiratory Exchange Ratio series	0.89693860
XTOT	0.53221684
XAMB	0.74846549
YTOT	0.81253476
YAMB	0.97195035
ZTOT	0.77206863
ZAMB	0.97857220
Oxygen consumption series	0.35523173
Carbon dioxide production series	0.37174720
Heat production (metabolic rate) series	0.25463657
Total food intake series	0.87256211
Total water intake series	0.25843296
XTOT series	0.31645068
XAMB series	0.51624614
YTOT series	0.56658559
YAMB series	0.86103923
ZTOT series	0.61565301
ZAMB series	0.92472879