Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00064745
Body weight after experiment	0.00131074
Oxygen consumption	0.54109784
Carbon dioxide production	0.21046026
Heat production (metabolic rate)	0.06623797
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.77406978
Cumulative food intake	0.21789060
Respiratory Exchange Ratio	0.17551291
Total water intake	0.01766262
Cumulative water intake	0.00042681
Respiratory Exchange Ratio series	0.20056604
XTOT	0.05868071
XAMB	0.13983984
YTOT	0.02656017
YAMB	0.08611565
ZTOT	0.40910135
ZAMB	0.37543058
Oxygen consumption series	0.45725946
Carbon dioxide production series	0.26444097
Heat production (metabolic rate) series	0.11640245
Total food intake series	0.52516476
Total water intake series	0.22045353
XTOT series	0.04167529
XAMB series	0.11819033
YTOT series	0.01316053
YAMB series	0.05013514
ZTOT series	0.61187674
ZAMB series	0.70250186