Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00478912
Body weight after experiment	0.00874236
Oxygen consumption	0.60285938
Carbon dioxide production	0.77880327
Heat production (metabolic rate)	0.01967837
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.27567891
Cumulative food intake	0.04365129
Respiratory Exchange Ratio	0.00708397
Total water intake	0.20982764
Cumulative water intake	0.54680041
Respiratory Exchange Ratio series	0.00199165
XTOT	0.15450007
XAMB	0.27912959
YTOT	0.32915205
YAMB	0.53064629
ZTOT	0.42602983
ZAMB	0.58649360
Oxygen consumption series	0.46780944
Carbon dioxide production series	0.77022404
Heat production (metabolic rate) series	0.03309272
Total food intake series	0.95500413
Total water intake series	0.68496153
XTOT series	0.00098278
XAMB series	0.00429993
YTOT series	0.06223332
YAMB series	0.13635995
ZTOT series	0.15567095
ZAMB series	0.70278603