Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.51901396
Body weight after experiment	0.32566219
Oxygen consumption	0.00003034
Carbon dioxide production	0.00021291
Heat production (metabolic rate)	0.00003696
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.75358594
Cumulative food intake	0.26644307
Respiratory Exchange Ratio	0.00320070
Total water intake	0.29999240
Cumulative water intake	0.16871280
Respiratory Exchange Ratio series	0.00042457
XTOT	0.00354513
XAMB	0.00046425
YTOT	0.00258764
YAMB	0.00069728
ZTOT	0.01036237
ZAMB	0.00469996
Oxygen consumption series	0.00000096
Carbon dioxide production series	0.00009217
Heat production (metabolic rate) series	0.00000455
Total food intake series	0.10571333
Total water intake series	0.01871268
XTOT series	0.00738501
XAMB series	0.00096056
YTOT series	0.00514620
YAMB series	0.00112744
ZTOT series	0.01403490
ZAMB series	0.01069160