Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.41221912
Body weight after experiment	0.24300815
Oxygen consumption	0.29533207
Carbon dioxide production	0.15752203
Heat production (metabolic rate)	0.13485350
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.75358594
Cumulative food intake	0.23134392
Respiratory Exchange Ratio	0.02323275
Total water intake	0.68902388
Cumulative water intake	0.50087969
Respiratory Exchange Ratio series	0.02015131
XTOT	0.69100690
XAMB	0.84115633
YTOT	0.79187297
YAMB	0.72135694
ZTOT	0.92662522
ZAMB	0.77792856
Oxygen consumption series	0.33952407
Carbon dioxide production series	0.11071871
Heat production (metabolic rate) series	0.13780113
Total food intake series	0.47496682
Total water intake series	0.50391961
XTOT series	0.78350057
XAMB series	0.70087402
YTOT series	0.61665619
YAMB series	0.61953818
ZTOT series	0.78677598
ZAMB series	0.90329150