Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.21113483
Body weight after experiment	0.06894629
Oxygen consumption	0.00308986
Carbon dioxide production	0.03356840
Heat production (metabolic rate)	0.02160348
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.13604699
Cumulative food intake	0.32608401
Respiratory Exchange Ratio	0.02416616
Total water intake	0.36679228
Cumulative water intake	0.17027260
Respiratory Exchange Ratio series	0.02005676
XTOT	0.85685963
XAMB	0.83463152
YTOT	0.81512701
YAMB	0.88153703
ZTOT	0.91333290
ZAMB	0.95181098
Oxygen consumption series	0.00817512
Carbon dioxide production series	0.05674861
Heat production (metabolic rate) series	0.04296660
Total food intake series	0.97290038
Total water intake series	0.86217561
XTOT series	0.86743998
XAMB series	0.69833777
YTOT series	0.82209928
YAMB series	0.68165664
ZTOT series	0.77379108
ZAMB series	0.80657405