Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.18438411
Body weight after experiment	0.01227184
Oxygen consumption	0.16511501
Carbon dioxide production	0.13516730
Heat production (metabolic rate)	0.03038837
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.25157033
Cumulative food intake	0.18590000
Respiratory Exchange Ratio	0.89715276
Total water intake	0.00089913
Cumulative water intake	0.00030423
Respiratory Exchange Ratio series	0.90332476
XTOT	0.30357600
XAMB	0.65203792
YTOT	0.22150758
YAMB	0.64759236
ZTOT	0.72889223
ZAMB	0.77150035
Oxygen consumption series	0.09859486
Carbon dioxide production series	0.11587405
Heat production (metabolic rate) series	0.00874041
Total food intake series	0.41179285
Total water intake series	0.00000054
XTOT series	0.22028022
XAMB series	0.62394312
YTOT series	0.22542807
YAMB series	0.71911780
ZTOT series	0.99440352
ZAMB series	0.86256047