Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.55133248
Body weight after experiment	0.65672692
Oxygen consumption	0.02744171
Carbon dioxide production	0.06481149
Heat production (metabolic rate)	0.00768431
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.07326770
Cumulative food intake	0.03399068
Respiratory Exchange Ratio	0.17497846
Total water intake	0.01924235
Cumulative water intake	0.00335123
Respiratory Exchange Ratio series	0.15963315
XTOT	0.14437626
XAMB	0.15767835
YTOT	0.29425337
YAMB	0.19788219
ZTOT	0.38301250
ZAMB	0.14416422
Oxygen consumption series	0.00380279
Carbon dioxide production series	0.02396861
Heat production (metabolic rate) series	0.00072202
Total food intake series	0.87573281
Total water intake series	0.20983405
XTOT series	0.20349001
XAMB series	0.18922347
YTOT series	0.28848435
YAMB series	0.16968288
ZTOT series	0.74317425
ZAMB series	0.31594024