Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.31650290
Body weight after experiment	0.24997620
Oxygen consumption	0.69891543
Carbon dioxide production	0.60513121
Heat production (metabolic rate)	0.75419586
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.32032854
Cumulative food intake	0.03086630
Respiratory Exchange Ratio	0.31544846
Total water intake	0.20858362
Cumulative water intake	0.89211704
Respiratory Exchange Ratio series	0.34382837
XTOT	0.39064810
XAMB	0.38697122
YTOT	0.81253476
YAMB	0.78233689
ZTOT	0.21470709
ZAMB	0.27520827
Oxygen consumption series	0.85597857
Carbon dioxide production series	0.63919787
Heat production (metabolic rate) series	0.63377807
Total food intake series	0.20013653
Total water intake series	0.31066062
XTOT series	0.18453736
XAMB series	0.13592012
YTOT series	0.76153828
YAMB series	0.59424573
ZTOT series	0.23803123
ZAMB series	0.24887293