Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.89999049
Body weight after experiment	0.93958011
Oxygen consumption	0.00102028
Carbon dioxide production	0.00304767
Heat production (metabolic rate)	0.00042815
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.74520104
Cumulative food intake	0.74382739
Respiratory Exchange Ratio	0.39754119
Total water intake	0.87767134
Cumulative water intake	0.11022876
Respiratory Exchange Ratio series	0.43124815
XTOT	0.32938237
XAMB	0.98847116
YTOT	0.44808451
YAMB	0.87624371
ZTOT	0.41100640
ZAMB	0.83036894
Oxygen consumption series	0.00038489
Carbon dioxide production series	0.00158459
Heat production (metabolic rate) series	0.00023510
Total food intake series	0.54591673
Total water intake series	0.42353663
XTOT series	0.43994563
XAMB series	0.97322327
YTOT series	0.50669136
YAMB series	0.78820342
ZTOT series	0.82525377
ZAMB series	0.75477762