Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.05086675
Body weight after experiment	0.07052634
Oxygen consumption	0.12304527
Carbon dioxide production	0.28719832
Heat production (metabolic rate)	0.73519174
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.10816324
Cumulative food intake	0.52901667
Respiratory Exchange Ratio	0.73327406
Total water intake	0.15661832
Cumulative water intake	0.58057165
Respiratory Exchange Ratio series	0.68072902
XTOT	0.25191783
XAMB	0.32117198
YTOT	0.62105474
YAMB	0.66452957
ZTOT	0.34327873
ZAMB	0.16865955
Oxygen consumption series	0.19911738
Carbon dioxide production series	0.28246675
Heat production (metabolic rate) series	0.85615345
Total food intake series	0.82505895
Total water intake series	0.21697656
XTOT series	0.28829402
XAMB series	0.45852308
YTOT series	0.54386578
YAMB series	0.70557256
ZTOT series	0.27714183
ZAMB series	0.26781597