Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.17903123
Body weight after experiment	0.01076047
Oxygen consumption	0.13225213
Carbon dioxide production	0.21914710
Heat production (metabolic rate)	0.42641750
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.01006966
Cumulative food intake	0.00251411
Respiratory Exchange Ratio	0.69569806
Total water intake	0.00065587
Cumulative water intake	0.00020282
Respiratory Exchange Ratio series	0.69184282
XTOT	0.18703668
XAMB	0.15669843
YTOT	0.26744705
YAMB	0.23782226
ZTOT	0.44615006
ZAMB	0.89051516
Oxygen consumption series	0.19965586
Carbon dioxide production series	0.23959684
Heat production (metabolic rate) series	0.47849653
Total food intake series	0.15204998
Total water intake series	0.01352549
XTOT series	0.28078751
XAMB series	0.15065362
YTOT series	0.24272108
YAMB series	0.14433598
ZTOT series	0.94473816
ZAMB series	0.52322675