Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.46841204
Body weight after experiment	0.63585937
Oxygen consumption	0.00248116
Carbon dioxide production	0.00137629
Heat production (metabolic rate)	0.00150017
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.43110369
Cumulative food intake	0.80898861
Respiratory Exchange Ratio	0.03647032
Total water intake	0.65724914
Cumulative water intake	0.99732806
Respiratory Exchange Ratio series	0.00454699
XTOT	0.18481428
XAMB	0.60231243
YTOT	0.64963225
YAMB	0.59093584
ZTOT	0.70139640
ZAMB	0.30742410
Oxygen consumption series	0.00000388
Carbon dioxide production series	0.00000004
Heat production (metabolic rate) series	0.00001557
Total food intake series	0.79540545
Total water intake series	0.91110282
XTOT series	0.20062503
XAMB series	0.68755419
YTOT series	0.67596131
YAMB series	0.54010645
ZTOT series	0.56572466
ZAMB series	0.20188605