Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.38020819
Body weight after experiment	0.36043373
Oxygen consumption	0.20015797
Carbon dioxide production	0.24974155
Heat production (metabolic rate)	0.07546092
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.83593467
Cumulative food intake	0.77430476
Respiratory Exchange Ratio	0.83904620
Total water intake	0.24526415
Cumulative water intake	0.03226372
Respiratory Exchange Ratio series	0.87041166
XTOT	0.45155382
XAMB	0.34974166
YTOT	0.38337504
YAMB	0.21227427
ZTOT	0.88158101
ZAMB	0.88914061
Oxygen consumption series	0.24565841
Carbon dioxide production series	0.25179402
Heat production (metabolic rate) series	0.10485900
Total food intake series	0.54196945
Total water intake series	0.57763124
XTOT series	0.57317997
XAMB series	0.35511476
YTOT series	0.39667745
YAMB series	0.20980224
ZTOT series	0.61582352
ZAMB series	0.58094443