Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.75943852
Body weight after experiment	0.32353585
Oxygen consumption	0.25393955
Carbon dioxide production	0.19363714
Heat production (metabolic rate)	0.13805249
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.73286791
Cumulative food intake	0.61759542
Respiratory Exchange Ratio	0.32244556
Total water intake	0.00712560
Cumulative water intake	0.00234840
Respiratory Exchange Ratio series	0.21894851
XTOT	0.34946793
XAMB	0.26123849
YTOT	0.08551951
YAMB	0.03088681
ZTOT	0.27966385
ZAMB	0.08858035
Oxygen consumption series	0.34021719
Carbon dioxide production series	0.19951734
Heat production (metabolic rate) series	0.21912102
Total food intake series	0.31923216
Total water intake series	0.02394185
XTOT series	0.52541546
XAMB series	0.30805573
YTOT series	0.11357607
YAMB series	0.03588161
ZTOT series	0.23231659
ZAMB series	0.08536566