Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.03376888
Body weight after experiment	0.67911729
Oxygen consumption	0.00371115
Carbon dioxide production	0.50835399
Heat production (metabolic rate)	0.00114054
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.03572550
Cumulative food intake	0.04685972
Respiratory Exchange Ratio	0.00001344
Total water intake	0.08279894
Cumulative water intake	0.02075908
Respiratory Exchange Ratio series	0.00000000
XTOT	0.39434613
XAMB	0.23570663
YTOT	0.16365560
YAMB	0.19211336
ZTOT	0.21719242
ZAMB	0.64313663
Oxygen consumption series	0.00810396
Carbon dioxide production series	0.51327783
Heat production (metabolic rate) series	0.00127412
Total food intake series	0.58699497
Total water intake series	0.20289791
XTOT series	0.41572485
XAMB series	0.21737728
YTOT series	0.16053444
YAMB series	0.12350530
ZTOT series	0.13428866
ZAMB series	0.98051470