Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.03035896
Body weight after experiment	0.17743518
Oxygen consumption	0.43032278
Carbon dioxide production	0.09062368
Heat production (metabolic rate)	0.93727403
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00052402
Cumulative food intake	0.00003233
Respiratory Exchange Ratio	0.00954493
Total water intake	0.00263657
Cumulative water intake	0.00055178
Respiratory Exchange Ratio series	0.00868363
XTOT	0.00009175
XAMB	0.00009967
YTOT	0.00143959
YAMB	0.00060920
ZTOT	0.03590380
ZAMB	0.02448444
Oxygen consumption series	0.37359389
Carbon dioxide production series	0.08699149
Heat production (metabolic rate) series	0.97231649
Total food intake series	0.14784583
Total water intake series	0.07365327
XTOT series	0.00000001
XAMB series	0.00000000
YTOT series	0.00021972
YAMB series	0.00000693
ZTOT series	0.06094534
ZAMB series	0.01930362