Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.73530362
Body weight after experiment	0.97197949
Oxygen consumption	0.00055754
Carbon dioxide production	0.00350800
Heat production (metabolic rate)	0.00121997
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.82047282
Cumulative food intake	0.93488744
Respiratory Exchange Ratio	0.12016920
Total water intake	0.51680212
Cumulative water intake	0.37393582
Respiratory Exchange Ratio series	0.07970388
XTOT	0.50856330
XAMB	0.41840790
YTOT	0.11862433
YAMB	0.09571782
ZTOT	0.69301682
ZAMB	0.55152107
Oxygen consumption series	0.00007011
Carbon dioxide production series	0.00081951
Heat production (metabolic rate) series	0.00010364
Total food intake series	0.37251725
Total water intake series	0.98808541
XTOT series	0.35963034
XAMB series	0.33539284
YTOT series	0.09657774
YAMB series	0.12845494
ZTOT series	0.87110795
ZAMB series	0.43501531