Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.24787693
Body weight after experiment	0.30749379
Oxygen consumption	0.39406626
Carbon dioxide production	0.84823543
Heat production (metabolic rate)	0.20199512
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.56816659
Cumulative food intake	0.76252724
Respiratory Exchange Ratio	0.19848683
Total water intake	0.76056445
Cumulative water intake	0.92529500
Respiratory Exchange Ratio series	0.14484595
XTOT	0.03173150
XAMB	0.06569864
YTOT	0.09489526
YAMB	0.17236910
ZTOT	0.17448486
ZAMB	0.23728667
Oxygen consumption series	0.51045823
Carbon dioxide production series	0.80247417
Heat production (metabolic rate) series	0.27108898
Total food intake series	0.60222533
Total water intake series	0.82523552
XTOT series	0.00862836
XAMB series	0.01997653
YTOT series	0.02199357
YAMB series	0.06643125
ZTOT series	0.00307340
ZAMB series	0.03649571