Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00250481
Body weight after experiment	0.02046137
Oxygen consumption	0.12470138
Carbon dioxide production	0.00909091
Heat production (metabolic rate)	0.84722385
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.46611204
Cumulative food intake	0.17634343
Respiratory Exchange Ratio	0.00222335
Total water intake	0.96672965
Cumulative water intake	0.35363726
Respiratory Exchange Ratio series	0.00033849
XTOT	0.45393866
XAMB	0.93998978
YTOT	0.78150572
YAMB	0.44445702
ZTOT	0.08363318
ZAMB	0.65843791
Oxygen consumption series	0.19565477
Carbon dioxide production series	0.01921046
Heat production (metabolic rate) series	0.77760117
Total food intake series	0.31359062
Total water intake series	0.74744199
XTOT series	0.53726910
XAMB series	0.76935306
YTOT series	0.84157831
YAMB series	0.39768146
ZTOT series	0.23518990
ZAMB series	0.93791481