Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00031331
Body weight after experiment	0.00212669
Oxygen consumption	0.09112823
Carbon dioxide production	0.03547896
Heat production (metabolic rate)	0.10935500
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00002055
Cumulative food intake	0.00778976
Respiratory Exchange Ratio	0.00000029
Total water intake	0.00002508
Cumulative water intake	0.00151776
Respiratory Exchange Ratio series	0.00000000
XTOT	0.00000140
XAMB	0.00001045
YTOT	0.00000101
YAMB	0.00000493
ZTOT	0.00002006
ZAMB	0.00017010
Oxygen consumption series	0.04738215
Carbon dioxide production series	0.04519806
Heat production (metabolic rate) series	0.15202342
Total food intake series	0.00030604
Total water intake series	0.00000003
XTOT series	0.00000000
XAMB series	0.00000041
YTOT series	0.00000000
YAMB series	0.00000011
ZTOT series	0.00006590
ZAMB series	0.00055494