Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00011734
Body weight after experiment	0.00196971
Oxygen consumption	0.00023357
Carbon dioxide production	0.00029039
Heat production (metabolic rate)	0.03202959
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.65777962
Cumulative food intake	0.01216923
Respiratory Exchange Ratio	0.74795076
Total water intake	0.33174092
Cumulative water intake	0.02923834
Respiratory Exchange Ratio series	0.68094728
XTOT	0.01678266
XAMB	0.01745479
YTOT	0.00735276
YAMB	0.01838751
ZTOT	0.63518508
ZAMB	0.93344382
Oxygen consumption series	0.00008016
Carbon dioxide production series	0.00019719
Heat production (metabolic rate) series	0.04801851
Total food intake series	0.33864429
Total water intake series	0.22347099
XTOT series	0.03425092
XAMB series	0.02211601
YTOT series	0.01352884
YAMB series	0.02627099
ZTOT series	0.93129190
ZAMB series	0.64842778