Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.20757150
Body weight after experiment	0.04568896
Oxygen consumption	0.10398449
Carbon dioxide production	0.40695074
Heat production (metabolic rate)	0.32320541
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00445935
Cumulative food intake	0.00065908
Respiratory Exchange Ratio	0.12577855
Total water intake	0.00144070
Cumulative water intake	0.00026723
Respiratory Exchange Ratio series	0.07557598
XTOT	0.79187297
XAMB	0.58153757
YTOT	0.68608809
YAMB	0.48086018
ZTOT	0.39779333
ZAMB	0.31222235
Oxygen consumption series	0.17612698
Carbon dioxide production series	0.44137160
Heat production (metabolic rate) series	0.48281866
Total food intake series	0.18798141
Total water intake series	0.00537163
XTOT series	0.83721068
XAMB series	0.47240434
YTOT series	0.68268747
YAMB series	0.36891816
ZTOT series	0.33671368
ZAMB series	0.22551537