Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.03460074
Body weight after experiment	0.13076689
Oxygen consumption	0.50408056
Carbon dioxide production	0.34890899
Heat production (metabolic rate)	0.54442809
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.40316258
Cumulative food intake	0.22744128
Respiratory Exchange Ratio	0.74087002
Total water intake	0.26407104
Cumulative water intake	0.15564092
Respiratory Exchange Ratio series	0.77141639
XTOT	0.18592303
XAMB	0.30200874
YTOT	0.53441191
YAMB	0.54331687
ZTOT	0.37755758
ZAMB	0.91843924
Oxygen consumption series	0.33846259
Carbon dioxide production series	0.31746799
Heat production (metabolic rate) series	0.71206590
Total food intake series	0.86989669
Total water intake series	0.58270547
XTOT series	0.22159323
XAMB series	0.45317670
YTOT series	0.50367701
YAMB series	0.60032785
ZTOT series	0.21343879
ZAMB series	0.83204569