Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00002379
Body weight after experiment	0.00008262
Oxygen consumption	0.00006891
Carbon dioxide production	0.00024613
Heat production (metabolic rate)	0.21969837
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.51483301
Cumulative food intake	0.44198898
Respiratory Exchange Ratio	0.98526745
Total water intake	0.29610540
Cumulative water intake	0.03976754
Respiratory Exchange Ratio series	0.92878872
XTOT	0.00020105
XAMB	0.00100138
YTOT	0.00023543
YAMB	0.00099528
ZTOT	0.03277311
ZAMB	0.04612139
Oxygen consumption series	0.00000003
Carbon dioxide production series	0.00000012
Heat production (metabolic rate) series	0.15697610
Total food intake series	0.60451942
Total water intake series	0.67855182
XTOT series	0.00000002
XAMB series	0.00000092
YTOT series	0.00000023
YAMB series	0.00000438
ZTOT series	0.04327530
ZAMB series	0.06120817