Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.05848516
Body weight after experiment	0.12731907
Oxygen consumption	0.91598954
Carbon dioxide production	0.36290236
Heat production (metabolic rate)	0.16871280
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00529855
Cumulative food intake	0.01119678
Respiratory Exchange Ratio	0.01291386
Total water intake	0.00389361
Cumulative water intake	0.00973045
Respiratory Exchange Ratio series	0.01666017
XTOT	0.06140936
XAMB	0.18151742
YTOT	0.12772091
YAMB	0.18984079
ZTOT	0.00219521
ZAMB	0.00309796
Oxygen consumption series	0.99822722
Carbon dioxide production series	0.46161943
Heat production (metabolic rate) series	0.16974662
Total food intake series	0.22295449
Total water intake series	0.00314788
XTOT series	0.03218422
XAMB series	0.10761693
YTOT series	0.07812296
YAMB series	0.12893589
ZTOT series	0.00062603
ZAMB series	0.01479418