Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.10237595
Body weight after experiment	0.05891116
Oxygen consumption	0.86324981
Carbon dioxide production	0.91788825
Heat production (metabolic rate)	0.06028961
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.01086100
Cumulative food intake	0.06199536
Respiratory Exchange Ratio	0.50114494
Total water intake	0.05772690
Cumulative water intake	0.09826942
Respiratory Exchange Ratio series	0.46544305
XTOT	0.62577966
XAMB	0.89107032
YTOT	0.74088310
YAMB	0.41291279
ZTOT	0.66696348
ZAMB	0.78308212
Oxygen consumption series	0.90959649
Carbon dioxide production series	0.90992713
Heat production (metabolic rate) series	0.02135404
Total food intake series	0.37945718
Total water intake series	0.29318276
XTOT series	0.63704245
XAMB series	0.81034118
YTOT series	0.57600319
YAMB series	0.31041595
ZTOT series	0.42473304
ZAMB series	0.52553408