Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.24058518
Body weight after experiment	0.61287488
Oxygen consumption	0.00698731
Carbon dioxide production	0.04396494
Heat production (metabolic rate)	0.00191795
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.09191100
Cumulative food intake	0.08840579
Respiratory Exchange Ratio	0.02437795
Total water intake	0.05271192
Cumulative water intake	0.00430583
Respiratory Exchange Ratio series	0.01998526
XTOT	0.18261149
XAMB	0.35310278
YTOT	0.16517626
YAMB	0.26264159
ZTOT	0.40342361
ZAMB	0.71690633
Oxygen consumption series	0.00727185
Carbon dioxide production series	0.05161001
Heat production (metabolic rate) series	0.00158844
Total food intake series	0.97862197
Total water intake series	0.14035812
XTOT series	0.16531179
XAMB series	0.28213428
YTOT series	0.09250526
YAMB series	0.20582346
ZTOT series	0.46596169
ZAMB series	0.60670870