Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00496639
Body weight after experiment	0.00268964
Oxygen consumption	0.31584491
Carbon dioxide production	0.84839571
Heat production (metabolic rate)	0.31107732
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.49208144
Cumulative food intake	0.40745245
Respiratory Exchange Ratio	0.00980222
Total water intake	0.11432866
Cumulative water intake	0.04527465
Respiratory Exchange Ratio series	0.00544950
XTOT	0.60926004
XAMB	0.62320103
YTOT	0.89596977
YAMB	0.82395981
ZTOT	0.76424061
ZAMB	0.50344428
Oxygen consumption series	0.26220234
Carbon dioxide production series	0.91061420
Heat production (metabolic rate) series	0.35421660
Total food intake series	0.33598386
Total water intake series	0.00072873
XTOT series	0.52850239
XAMB series	0.54034410
YTOT series	0.91175533
YAMB series	0.76777438
ZTOT series	0.47454838
ZAMB series	0.32676354