Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.57899950
Body weight after experiment	0.59144534
Oxygen consumption	0.01352148
Carbon dioxide production	0.04539288
Heat production (metabolic rate)	0.00831891
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.13916072
Cumulative food intake	0.33864204
Respiratory Exchange Ratio	0.33442197
Total water intake	0.06237787
Cumulative water intake	0.17823399
Respiratory Exchange Ratio series	0.40449246
XTOT	0.16466819
XAMB	0.13894587
YTOT	0.15572313
YAMB	0.18646915
ZTOT	0.52351014
ZAMB	0.33358204
Oxygen consumption series	0.02897906
Carbon dioxide production series	0.07329270
Heat production (metabolic rate) series	0.01501091
Total food intake series	0.97883182
Total water intake series	0.30246174
XTOT series	0.12403415
XAMB series	0.15048494
YTOT series	0.10976853
YAMB series	0.13889369
ZTOT series	0.29265193
ZAMB series	0.56626548