Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.82257827
Body weight after experiment	0.17040110
Oxygen consumption	0.00015739
Carbon dioxide production	0.00299823
Heat production (metabolic rate)	0.00025199
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.88786224
Cumulative food intake	0.74087002
Respiratory Exchange Ratio	0.00283851
Total water intake	0.52024540
Cumulative water intake	0.47359595
Respiratory Exchange Ratio series	0.00076064
XTOT	0.46039666
XAMB	0.51266881
YTOT	0.07723009
YAMB	0.13927677
ZTOT	0.72637751
ZAMB	0.93043961
Oxygen consumption series	0.00011021
Carbon dioxide production series	0.00438583
Heat production (metabolic rate) series	0.00015818
Total food intake series	0.25824686
Total water intake series	0.07062614
XTOT series	0.61986615
XAMB series	0.81218649
YTOT series	0.05212475
YAMB series	0.10929133
ZTOT series	0.99591476
ZAMB series	0.65590282