Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00507834
Body weight after experiment	0.05352281
Oxygen consumption	0.00001928
Carbon dioxide production	0.00000359
Heat production (metabolic rate)	0.00447195
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00181569
Cumulative food intake	0.00038735
Respiratory Exchange Ratio	0.00001008
Total water intake	0.00069332
Cumulative water intake	0.00000567
Respiratory Exchange Ratio series	0.00000001
XTOT	0.01584648
XAMB	0.11593946
YTOT	0.03102197
YAMB	0.24517079
ZTOT	0.01668633
ZAMB	0.02645205
Oxygen consumption series	0.00000001
Carbon dioxide production series	0.00000000
Heat production (metabolic rate) series	0.00005805
Total food intake series	0.16003114
Total water intake series	0.00771296
XTOT series	0.01343389
XAMB series	0.10615932
YTOT series	0.01701542
YAMB series	0.26756448
ZTOT series	0.00959285
ZAMB series	0.06370900