Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00593557
Body weight after experiment	0.10115340
Oxygen consumption	0.02863811
Carbon dioxide production	0.14416422
Heat production (metabolic rate)	0.00098349
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.74849133
Cumulative food intake	0.21415219
Respiratory Exchange Ratio	0.05761768
Total water intake	0.07081363
Cumulative water intake	0.00428319
Respiratory Exchange Ratio series	0.06270008
XTOT	0.77647114
XAMB	0.51698025
YTOT	0.63527558
YAMB	0.40531093
ZTOT	0.87625483
ZAMB	0.91977174
Oxygen consumption series	0.06019616
Carbon dioxide production series	0.18727604
Heat production (metabolic rate) series	0.00164846
Total food intake series	0.53576075
Total water intake series	0.36290159
XTOT series	0.77210936
XAMB series	0.39576448
YTOT series	0.64833707
YAMB series	0.32044776
ZTOT series	0.70032519
ZAMB series	0.89724650