Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.06702815
Body weight after experiment	0.13878895
Oxygen consumption	0.18424611
Carbon dioxide production	0.02535101
Heat production (metabolic rate)	0.46230367
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.43405688
Cumulative food intake	0.66372209
Respiratory Exchange Ratio	0.00063128
Total water intake	0.78877399
Cumulative water intake	0.99732806
Respiratory Exchange Ratio series	0.00030585
XTOT	0.80477045
XAMB	0.64723023
YTOT	0.71081105
YAMB	0.60021927
ZTOT	0.11434678
ZAMB	0.03139127
Oxygen consumption series	0.20185987
Carbon dioxide production series	0.02136788
Heat production (metabolic rate) series	0.61206759
Total food intake series	0.25509320
Total water intake series	0.93243054
XTOT series	0.84519418
XAMB series	0.89545367
YTOT series	0.82320593
YAMB series	0.82298298
ZTOT series	0.16364864
ZAMB series	0.06176494