Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.07739307
Body weight after experiment	0.77528785
Oxygen consumption	0.07346567
Carbon dioxide production	0.93310861
Heat production (metabolic rate)	0.02198826
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.02459134
Cumulative food intake	0.00414124
Respiratory Exchange Ratio	0.00007762
Total water intake	0.27422740
Cumulative water intake	0.00178157
Respiratory Exchange Ratio series	0.00000110
XTOT	0.02174761
XAMB	0.10594782
YTOT	0.11034677
YAMB	0.18646915
ZTOT	0.03680064
ZAMB	0.10345560
Oxygen consumption series	0.11100887
Carbon dioxide production series	0.94272223
Heat production (metabolic rate) series	0.03132634
Total food intake series	0.58685561
Total water intake series	0.67371625
XTOT series	0.02677755
XAMB series	0.16377241
YTOT series	0.14691002
YAMB series	0.32747484
ZTOT series	0.11666979
ZAMB series	0.24219362