Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.94910406
Body weight after experiment	0.45920415
Oxygen consumption	0.06623797
Carbon dioxide production	0.14788892
Heat production (metabolic rate)	0.03605196
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.12744672
Cumulative food intake	0.66128667
Respiratory Exchange Ratio	0.44398489
Total water intake	0.16511501
Cumulative water intake	0.16716375
Respiratory Exchange Ratio series	0.49297628
XTOT	0.37613091
XAMB	0.55840302
YTOT	0.59790461
YAMB	0.94225667
ZTOT	0.47108394
ZAMB	0.43286769
Oxygen consumption series	0.01126870
Carbon dioxide production series	0.02614125
Heat production (metabolic rate) series	0.00362432
Total food intake series	0.66233148
Total water intake series	0.55779813
XTOT series	0.41090884
XAMB series	0.73354944
YTOT series	0.56874761
YAMB series	0.85970996
ZTOT series	0.57564124
ZAMB series	0.82428376