Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.97519170
Body weight after experiment	0.42174085
Oxygen consumption	0.02496052
Carbon dioxide production	0.01761257
Heat production (metabolic rate)	0.01076915
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.01698217
Cumulative food intake	0.00306438
Respiratory Exchange Ratio	0.39285464
Total water intake	0.03502566
Cumulative water intake	0.03944596
Respiratory Exchange Ratio series	0.47071389
XTOT	0.12197594
XAMB	0.22917462
YTOT	0.08240011
YAMB	0.28767313
ZTOT	0.59093584
ZAMB	0.94112000
Oxygen consumption series	0.00574885
Carbon dioxide production series	0.00413313
Heat production (metabolic rate) series	0.00200900
Total food intake series	0.61854361
Total water intake series	0.16492859
XTOT series	0.10294676
XAMB series	0.31988789
YTOT series	0.04834268
YAMB series	0.18526773
ZTOT series	0.62763169
ZAMB series	0.89208947