Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.88935464
Body weight after experiment	0.42470999
Oxygen consumption	0.14381091
Carbon dioxide production	0.08320140
Heat production (metabolic rate)	0.09772681
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00012262
Cumulative food intake	0.00192079
Respiratory Exchange Ratio	0.36734636
Total water intake	0.00005338
Cumulative water intake	0.00154403
Respiratory Exchange Ratio series	0.87799541
XTOT	0.06094742
XAMB	0.05980516
YTOT	0.06048837
YAMB	0.07564532
ZTOT	0.07675478
ZAMB	0.03112783
Oxygen consumption series	0.05191935
Carbon dioxide production series	0.05775084
Heat production (metabolic rate) series	0.02745059
Total food intake series	0.86502354
Total water intake series	0.55225762
XTOT series	0.01776985
XAMB series	0.01779761
YTOT series	0.01500445
YAMB series	0.02624878
ZTOT series	0.14513627
ZAMB series	0.09035362