Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.05676221
Body weight after experiment	0.69673121
Oxygen consumption	0.00007656
Carbon dioxide production	0.00017435
Heat production (metabolic rate)	0.00155294
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.55684069
Cumulative food intake	0.26572141
Respiratory Exchange Ratio	0.34119042
Total water intake	0.45519662
Cumulative water intake	0.03488110
Respiratory Exchange Ratio series	0.13310358
XTOT	0.01178413
XAMB	0.01167282
YTOT	0.02332766
YAMB	0.05271192
ZTOT	0.20377675
ZAMB	0.54339651
Oxygen consumption series	0.00000140
Carbon dioxide production series	0.00000122
Heat production (metabolic rate) series	0.00004324
Total food intake series	0.21654279
Total water intake series	0.05553052
XTOT series	0.01135995
XAMB series	0.02079251
YTOT series	0.01614848
YAMB series	0.05765973
ZTOT series	0.34360560
ZAMB series	0.85029600