Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.08103026
Body weight after experiment	0.06147650
Oxygen consumption	0.52133248
Carbon dioxide production	0.07140230
Heat production (metabolic rate)	0.88021598
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.02292865
Cumulative food intake	0.03073625
Respiratory Exchange Ratio	0.00137629
Total water intake	0.24049519
Cumulative water intake	0.08161884
Respiratory Exchange Ratio series	0.00018780
XTOT	0.02819661
XAMB	0.05691814
YTOT	0.06521018
YAMB	0.11206048
ZTOT	0.63560444
ZAMB	0.52353798
Oxygen consumption series	0.42531833
Carbon dioxide production series	0.04824493
Heat production (metabolic rate) series	0.77352277
Total food intake series	0.57158277
Total water intake series	0.77610211
XTOT series	0.01643515
XAMB series	0.03035333
YTOT series	0.06523007
YAMB series	0.12238759
ZTOT series	0.74787135
ZAMB series	0.67385026